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Mao, Xue
- Enhanced Oil Accumulation in Tobacco (Nicotiana tabacum L.) Leaves by Ectopic Overexpression of VgDGAT1a for Renewable Production of Biofuels
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Authors
Affiliations
1 Institute of Molecular Agriculture and Bioenergy, Shanxi Agricultural University, Taigu, Shanxi 030801, CN
2 Department of Life Science, Heze University, Heze, Shandong 274015, CN
1 Institute of Molecular Agriculture and Bioenergy, Shanxi Agricultural University, Taigu, Shanxi 030801, CN
2 Department of Life Science, Heze University, Heze, Shandong 274015, CN
Source
Current Science, Vol 114, No 06 (2018), Pagination: 1234-1240Abstract
To increase oil accumulation in the high-biomass vegetative organs of tobacco (Nicotiana tabacum L.) plants for renewable production of biofuels, VgDGAT1a isolated from developing seeds of Vernonia galamensis L. was ectopically overexpressed in tobacco leaves using a constitutive promoter. The transgenic tobacco leaves showed a 3.5–5.0-fold increase in oil content compared to the control, with a maximum increase of 9.2% (DW). The transgenic leaves also showed a substantial change in fatty acid composition, with significant enhancement of linoleic acid (18 : 2) and notable reduction of -linolenic acid (18 : 3). The overexpression of VgDGAT1a exhibited no deleterious effect on other phenotypes in the tobacco plant. These results will facilitate development of a novel system for lipid metabolic engineering in vegetative organs of plants, as well as provide a platform for the production of biofuels using the vegetative organs of commercial non-food crops.Keywords
Biofuels, Metabolic Engineering, Plant Oil, Tobacco Leaves, VgDGAT1a Gene.Full Text
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